In the first trial , combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis , and then a modified discontinuous percoll gradient centrifugation method ( 15 % , 22 % , 30 % , 40 % , 50 % , 60 % ) was introduced to isolate spermatids from the cellular suspensions . the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis , and the viability of spermatogenic cells was assessed by using eosin y exclusion test 在第一部分试验中，首先利用连续3次组合酶消化成年小鼠睾丸制备睾丸细胞悬液，然后经6层非连续percoll梯度离心法( 15 、 22 、 30 、 40 、 50和60 )分离，通过形态学和流式细胞术鉴定南京医科大学硕士学位论文各个percoll组分中精子细胞的含量，并以伊红y排斥试验测定细胞的存活率。
2.
In the second trial , this modified discontinuous percoll gradient centrifugation method was introduced to isolate spermatids from the semen of fifteen male infertile patients . then the effect was identified by wright - giemsa stain , flow cytometry analysis , immunocytochemistry and fluorescence in situ hybridization ( fish ) . similary , the 22 % percoll fraction contained mostly haploid cells [ ( 91 . 85 ? 5 . 18 ) % ] ( p < 0 . 005 ) and the mean density in this fraction was ( 1 . 010 ? 0 . 786 ) x 105 / ml C法，对15例各种类型不育患者的精液细胞进行分离，并利用瑞姬染色法、流式细胞术、免疫细胞化学和荧光原位杂交oisffi等方法，从细胞形态特征、 dna倍体、细胞表面标i己与分化抗原，以及原位杂交信号的数目和位置结合细胞核特有的形态等方面加以鉴定。
